DIRECT CHROMOSOME ANALYSIS AND FISH DETECTION OF PRIMARY GASTRIC CANCER
Abstract
Objective: To investigate chromosome aberrations and their role in the genesis and development of primary gastric cancer.
Methods: An improved, direct chromosome preparation from solid tumors was adopted for G-banding analysis followed by FISH on decolored G-banding chromosomes so that chromosome aberrations could be confirmed at DNA level.
Results: A total of 28 primary gastric cancer specimens were studies. Case I and case 2 had simple chromosome numerical changes: 49, XY, +2, +8, +9 and 48, +8, +20, respectively. All but case 1 and 2 had complicated chromosome abnormalities. Chromosome structural of frequent occurrence involved del(Tq)(21/26), del(3p)(14/26), delOp)01/26) and del(17p)(10/26). The chromosome abnormalities could be simple and complicated. In former, numerical changes involving 1 to 3 chromosome could be observed. Trisomies 8 and 9 might represent a cytogenetic subgroup of primary gastric cancer. In the later, the del(Tq) was the most consistent aberration. 7q32-qter was the commonly lost segment.
Conclusion: Numerical and structural alterations of chromosomes are present in primary gastric cancer. Del(Tq) is one of the structural change characteristic of primary gastric cancer. In the 7q32- qter fragment, a tumor suppressor gene probably exists and it may have close relation to the genesis and progression of gastric cancer.
Methods: An improved, direct chromosome preparation from solid tumors was adopted for G-banding analysis followed by FISH on decolored G-banding chromosomes so that chromosome aberrations could be confirmed at DNA level.
Results: A total of 28 primary gastric cancer specimens were studies. Case I and case 2 had simple chromosome numerical changes: 49, XY, +2, +8, +9 and 48, +8, +20, respectively. All but case 1 and 2 had complicated chromosome abnormalities. Chromosome structural of frequent occurrence involved del(Tq)(21/26), del(3p)(14/26), delOp)01/26) and del(17p)(10/26). The chromosome abnormalities could be simple and complicated. In former, numerical changes involving 1 to 3 chromosome could be observed. Trisomies 8 and 9 might represent a cytogenetic subgroup of primary gastric cancer. In the later, the del(Tq) was the most consistent aberration. 7q32-qter was the commonly lost segment.
Conclusion: Numerical and structural alterations of chromosomes are present in primary gastric cancer. Del(Tq) is one of the structural change characteristic of primary gastric cancer. In the 7q32- qter fragment, a tumor suppressor gene probably exists and it may have close relation to the genesis and progression of gastric cancer.