THE EXAMINATION OF AN EBV-DNASE GENE FRAGMENT IN THE PARAFFIN-EMBEDDED NPC, PRECANCEROUS AND FROM HIGHRISK POPULATION NASOPHARYNGEAL TISSUES
Abstract
Objective: To examine if the EB virus appears in nasopharyngeal epithelial cells in the course of tumorigenesis in nasopharyngeal carcinoma (NPC).
Methods: Based on the annual investigation of Sihui County, a cancer-prevention base of Sun Yat-sen University of Medical Sciences(SUMS) and Tumor Hospital out-patient clinic, more than 450 paraffinembedded NPC tissue specimens, precancerous lesions and precaneerosis from a high-risk population were collected for detection of an EBV-DNase geue fragment by an optimized PCR method.
Results: In 145 of the 149 (97.3%) cases with invasive NPC, and 2 of 4 cases with in situ NPC, only 2 of the 155 subjects with precancerosis was DNase gene positive, and all 47 cases examined in the out-patient department of the Tumor Hospital with precancerous lesions in the nasopharynx were negative.
Conclusion: The universal presence of an EBV-DNase gene in NPC tissues and the paucity of EBV-DNase gene in nonmalignant and precancerous lesions in the nasopharynx in a high-risk population imply that carcinogenesis of NPC occurs before the appearance of EBV in the nasopharynx.
Methods: Based on the annual investigation of Sihui County, a cancer-prevention base of Sun Yat-sen University of Medical Sciences(SUMS) and Tumor Hospital out-patient clinic, more than 450 paraffinembedded NPC tissue specimens, precancerous lesions and precaneerosis from a high-risk population were collected for detection of an EBV-DNase geue fragment by an optimized PCR method.
Results: In 145 of the 149 (97.3%) cases with invasive NPC, and 2 of 4 cases with in situ NPC, only 2 of the 155 subjects with precancerosis was DNase gene positive, and all 47 cases examined in the out-patient department of the Tumor Hospital with precancerous lesions in the nasopharynx were negative.
Conclusion: The universal presence of an EBV-DNase gene in NPC tissues and the paucity of EBV-DNase gene in nonmalignant and precancerous lesions in the nasopharynx in a high-risk population imply that carcinogenesis of NPC occurs before the appearance of EBV in the nasopharynx.