GENOMIC STRUCTURE OF MOUSE TBX2 AND DETECTION OF EXPRESSION OF TBX2 IN NORMAL AND MALIGNANCE MELANOPHORE BY RT-PCR
Abstract
Objective: Sequencing of mouse Tbx2 gone and observing the expression of Tbx2 gene in normal and malignant melanophore.
Methods: The PCR products of Tbx2 cDNA were cloned into pUC18 vector and sequenced. The normal and malignant melanocytes were used to extract total RNA. The expression of Tbx2 gene was detected by RT-PCR.
Results: The Tb×2 genome is composed of seven exons and six introns. No expression of Tbx2 gene in the normal melanocytes was noted, but all malignant melanocytes showed expression of Tb×2 gene.
Conclusion: The observation showed the analysis of the genomic structure of mouse Tbx2. Tbx2 plays a critical role during the development of the malignant melanophore.
Methods: The PCR products of Tbx2 cDNA were cloned into pUC18 vector and sequenced. The normal and malignant melanocytes were used to extract total RNA. The expression of Tbx2 gene was detected by RT-PCR.
Results: The Tb×2 genome is composed of seven exons and six introns. No expression of Tbx2 gene in the normal melanocytes was noted, but all malignant melanocytes showed expression of Tb×2 gene.
Conclusion: The observation showed the analysis of the genomic structure of mouse Tbx2. Tbx2 plays a critical role during the development of the malignant melanophore.