EXPRESSION OF MDR1, MRP AND LRP GENES IN GASTRIC CARCINOMA AND THEIR CLINICAL SIGNIFICANCE
Abstract
Objective: To explore the expression of mdrl, multidrug resistance-associated protein (MRP) and lung resistance protein (LRP) genes in human gastric cancer and their clinical significance.
Methods: The mdrl mRNA was assayed by RT-PCR, the MRP and LRP were detected by flow cytometry.
Results: The positive rate of mdrl mRNA was 44.4% (12/27), and the mean MRP and LRP expression were independent upon patient histologic type, nodal involvement, and TNM stage. The mdrl mRNA expression in patients with serosa invasion was 30.0% (6/20), much lower than that without serosa invasion (85.7%).
Conclusion: The multidrug resistance cells are present in primary gastric carcinomas prior to chemotherapy, and analysis of mdrl gene, MRP, LRP may have guiding significance in the treatment of gastric carcinoma.
Methods: The mdrl mRNA was assayed by RT-PCR, the MRP and LRP were detected by flow cytometry.
Results: The positive rate of mdrl mRNA was 44.4% (12/27), and the mean MRP and LRP expression were independent upon patient histologic type, nodal involvement, and TNM stage. The mdrl mRNA expression in patients with serosa invasion was 30.0% (6/20), much lower than that without serosa invasion (85.7%).
Conclusion: The multidrug resistance cells are present in primary gastric carcinomas prior to chemotherapy, and analysis of mdrl gene, MRP, LRP may have guiding significance in the treatment of gastric carcinoma.