THE RELATIONSHIP BETWEEN NON-HODGKIN'S LYMPHOMA AND THE GENE REARRANGEMENT
Abstract
Objective: To investigate the pattern of clonal rearrangement of immunoglobulin heavy chain gene (IGH) and T-cell receptor γ gene (TCRγ) of Non- Hodgkin's lymphoma (NHL).
Methods: Bone marrow smears of 211 patients of NHL were detected by PCR, the rearranged IGH and TCRγ gene was amplified using oligonucleotide primers.
Results: The clonal rearrangement of IGH gene was detectable in 51.2% (108/211); the clonal rearrangement of TCRγgene was detectable in 21.3% (45/211); both IGH and TCRγ was detectable in 5.7% (12/211); no clonal rearrangement in 21.8% (46/211). And compared clonal gene rearrangement with pathological type and primary site of tumor. Ten patients Of NHL were investigated serially. 5/10 patients still had clonal gene rearrangement at clinical complete remission.
Conclusion: It demonstrated that this assay may be useful in monitoring the minimal residual disease (MRD) and in evaluating effectiveness of therapy.
Methods: Bone marrow smears of 211 patients of NHL were detected by PCR, the rearranged IGH and TCRγ gene was amplified using oligonucleotide primers.
Results: The clonal rearrangement of IGH gene was detectable in 51.2% (108/211); the clonal rearrangement of TCRγgene was detectable in 21.3% (45/211); both IGH and TCRγ was detectable in 5.7% (12/211); no clonal rearrangement in 21.8% (46/211). And compared clonal gene rearrangement with pathological type and primary site of tumor. Ten patients Of NHL were investigated serially. 5/10 patients still had clonal gene rearrangement at clinical complete remission.
Conclusion: It demonstrated that this assay may be useful in monitoring the minimal residual disease (MRD) and in evaluating effectiveness of therapy.