EXPRESSION OF mdr-1 GENE IN CANCER TISSUE AND ITS ASSOCIATION WITH MORPHOLOGICAL INDEXES OF ESOPHAGEAL CARCINOMA IN ANYANG
Abstract
Objective: Overexpression of mdr-1 gene is associated with multidrug resistance (MDR) and aggressive characteristics of malignance. Our purpose was to detect the levels of P-gp expression in fresh untreated esophageal carcinomas, and to correlate these levels to current prognostic indicators of morphology.
Methods: Reverse transcription polymerase chain reaction (RT-PCR) was used to investigate mdr-I gene expression of 46 samples from untreated esophageal carcinoma, and compared the positive incidences among differentiated grades, TNM stages and macroscopic types.
Results: All 46 samples were pathologically squamous cell carcinoma. The positive, incidences of mdr-I gene expression were 37% (17/46) in whole group, 35% (6/17), 40% (8/20), 33% (3/9), for I, H and III differentiated grades, respectively. The expression rates of 33% (6/18), 40% ($/12), and 37% (6/16), were found in Ha, Hb, and HI stage of TNM, respectively. In macroscopic type view, the positive incidence was 37% (3/8) in constrietive, 33% (5/15) in fungating, 40% (6/14) in marrowlike, 33% (3/9) in uleerative type. There were no statistically significant differences among each eategory system of morphology.
Conclusion: The result, high level expression of mdr-1 gene in untreated esophageal carcinoma, suggested the poor efficacy of chemotherapy for some esophageal carcinoma patients. And we should cautiously choose cases who will receive chemotherapy. Surgery is still the best treatment for carcinoma of esophagus. Besides, the data also revealed that the expression of mdr-1 gene in untreated esophageal cancer was independent of morphologie prognostic indexes, and that there were no correlation between mdr-I gene expression and morphological indexes.
Methods: Reverse transcription polymerase chain reaction (RT-PCR) was used to investigate mdr-I gene expression of 46 samples from untreated esophageal carcinoma, and compared the positive incidences among differentiated grades, TNM stages and macroscopic types.
Results: All 46 samples were pathologically squamous cell carcinoma. The positive, incidences of mdr-I gene expression were 37% (17/46) in whole group, 35% (6/17), 40% (8/20), 33% (3/9), for I, H and III differentiated grades, respectively. The expression rates of 33% (6/18), 40% ($/12), and 37% (6/16), were found in Ha, Hb, and HI stage of TNM, respectively. In macroscopic type view, the positive incidence was 37% (3/8) in constrietive, 33% (5/15) in fungating, 40% (6/14) in marrowlike, 33% (3/9) in uleerative type. There were no statistically significant differences among each eategory system of morphology.
Conclusion: The result, high level expression of mdr-1 gene in untreated esophageal carcinoma, suggested the poor efficacy of chemotherapy for some esophageal carcinoma patients. And we should cautiously choose cases who will receive chemotherapy. Surgery is still the best treatment for carcinoma of esophagus. Besides, the data also revealed that the expression of mdr-1 gene in untreated esophageal cancer was independent of morphologie prognostic indexes, and that there were no correlation between mdr-I gene expression and morphological indexes.