By means of itamunohistological technique, the expressions of p53, c-erbB-2, EGFR and ras were examined on the adjacent sections of 75 cases of gastric cancer (GC), and their clinicopathological and prognostic significance were studied. Results: I) Overexpressions of p53 and c-erbB-2 were early and late sign of malignant change of gastric mucosa, respectively; EGFR and ras had close association with the degree of malignancy of GC. 2) The expression of ras had positive relationship with the expression of EGFR, and negative relationship with c-erbB-2.3). The multivariate analysis of Cox model of GC showed only DNA ploidy, expression of EGFR, lymph node metastasis and distant metastasis were independent prognostic factors of GC.